Semaphorin 4d, Peptidylarginine Deiminase 2 & Matrix Metalloproteinase

The current study was conducted to assess the levels of GCF SEMA 4D, PAD 2, and MMP 8 found to be increased in smokers having stage III periodontitis. A total of sixty subjects were enrolled and divided into three groups with 20 patients in each group. Group I included healthy individuals who showed no symptoms of periodontal disease, Group II included nonsmokers with stage III periodontitis, and Group III included smokers (patients with history of smoking at least 10 cigarettes per day for the last 3 years) with stage III periodontitis. All the subjects were assessed clinically and biochemically for categorization into respective groups. Clinical parameters evaluated were PI, GI, PBI, PPD, and CAL. A significantly high PPD and Smokers with chronic periodontitis had lower GI and PBI than nonsmokers having chronic periodontitis. Biochemical parameters included were SEMA 4D, PAD 2, MMP 8levels from GCF samples and ELISA kit was used to analyze the respective levels.