SCREENING OF RGA MARKER(s) IN INDIAN BEAN AGAINST YMD

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In present investigation six resistant (P-19- 169, V-19-15, V-19-35, GNIB-22, W-19-05, V-19-24) and six highly susceptible (P-19-176, W-19- 60, P-19-127, V-19-11, W-19-58, V-19-106) Indian bean genotypes were screened by nine pairs of RGA primers. Seven pairs of RGA primers were successfully amplified as a result of this in all resistant genotypes, but not in all highly susceptible genotypes. The amplicons were obtained in seven pairs of RGA primers, where five pairs of RGA primersRGA1FCG &RGA1R, VMYR1F & VMYR1R, YR4F & YR4R, RGAIB3 & RGAIB4, RGAIB5 & RGAIB6 amplified single band size of approximately 450 bp, 450 bp, 450 bp, 450 bp and 1050 bp respectively in resistant genotypes, while only two pairs of RGA primers CYR1F & CYR1R and RGAIB1 & RGAIB2 were found with prominent band of approximately 950bp and 350bp. According to the data from the current investigation, seven pairs of RGA markers have proven successful in differentiating between the resistant and highly susceptible genotypes of Indian beans. These RGA markers can be employed in investigations mapping resistance genes and validating markers with long-lasting YMV resistance.