Clonacion y Expresion de Proteinas Recombinantes

Varela Silva Jose Antonio,Ayala Lujan Jorge Luis,Jose Antonio Varela Silva,Varela Silva Jose Antonio,Ayala Lujan Jorge Luis

Clonacion y Expresion de Proteinas Recombinantes
Format
Paperback
Publisher
Eae Editorial Academia Espanola
Country
United States
Published
27 October 2011
Pages
80
ISBN
9783845498881

Clonacion y Expresion de Proteinas Recombinantes

Varela Silva Jose Antonio,Ayala Lujan Jorge Luis,Jose Antonio Varela Silva,Varela Silva Jose Antonio,Ayala Lujan Jorge Luis

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El presente texto fue escrito con la finalidad de dar a conocer algunos metodos de biologia molecular, los cuales en la actualidad se usan para la produccion y comercializacion de proteinas, entre otros usos. Nuestro ensayo se enfoco en la clonacion de la proteina Interferon Gamma Humano, para lo cual se utilizo un vector de clonacion disenado para bacterias y se expreso en E. coli, siendo este, un sistema heterologo al homo sapiens; logrando asi la expresion de esta proteina en bacterias E. coli, las cuales proporcionan un sistema eficiente de produccion de proteinas, debido a su alta taza de crecimiento y esto se refleja en un alto rendimiento en un corto espacio de tiempo.

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