Protein Biochemistry and Proteomics

7 ¼ X 10 ¼ in The Concept of the Experimenter Abbreviations
1. The Daily Bread
1.1 Making Buffers
1.2 P rotein Determination
1.3 Gels
1.4 Staining G els
1.5 Precipitate and Concentrating
1.6 Bl otting

1.7 Autoradiography of Gels and Blots
2. Ligand Binding
2.1 Radioactive Ligand Marking

2.2 Binding 2.3 Analysis of Binding Data
2.4 Crosslinking of Ligands 2.5 Purposes

1. Solubilization of Membrane Proteins
   3.1 Detergents
   3.2 Solubilization
   
2. Protein Detection via Func tional Measurements 4.1 Translocators
   4.2 Rec onstitution
   4.3 Flux Assay 4.4 Constructive T houghts
   
3. Cleaning and Purifying
   5.1 Pure F un
   5.2 Conventional Purification Methods
   5.3 Affinity Chromatography
   5.4 The Purity Test
   5.5 Profiting
   
4. Antibodies
   6.1 Production