Background: Systemic lupus erythematosus (SLE) is an autoimmune connective tissue disease characterised by a reduced immune tolerance of the body's immune system and an enormous production of autoantibodies, which ultimately cause damage to most of body tissues and organs, including the skin, blood and its early diagnosis is important to reduce complications.Aim of the work: This study aimed to investigate the possibility of a link between a single nucleotide polymorphism (SNP) in the IL-35 gene (rs4740) and susceptibility to SLE occurrence.Subjects and methodology: In this Observational study, 30 patients already diagnosed with SLE (Group 1) were compared to 20 apparently healthy subjects (Group 2) who were matched for age and sex (all were females) and who had no history of autoimmune disease. Following informed consent for scientific research from the Al-Azhar Ethical Committee for Scientific Research, patients and a control group were enrolled in this study Results: The findings revealed that there was no statistically significant difference in age between SLE patients and healthy controls in this study