Single-Molecule Studies of Nucleic Acids and Their Proteins
David Bensimon (Research Director and Professor, Research Director and Professor, LPS-ENS, PSL, CNRS and Department of Chemistry and Biochemistry, UCLA, USA),Vincent Croquette (Research Director, Research Director, LPS-ENS, PSL, CNRS, France),Jean-Francois Allemand (Professor, Professor, LPS-ENS, PSL, CNRS, France),Xavier Michalet (Researcher, Researcher, Department of Chemistry and Biochemistry, UCLA, USA),Terence Strick (Research Director and Principle Investigator, Research Director and Principle Investigator, IBENS, PSL, CNRS, France)
Single-Molecule Studies of Nucleic Acids and Their Proteins
David Bensimon (Research Director and Professor, Research Director and Professor, LPS-ENS, PSL, CNRS and Department of Chemistry and Biochemistry, UCLA, USA),Vincent Croquette (Research Director, Research Director, LPS-ENS, PSL, CNRS, France),Jean-Francois Allemand (Professor, Professor, LPS-ENS, PSL, CNRS, France),Xavier Michalet (Researcher, Researcher, Department of Chemistry and Biochemistry, UCLA, USA),Terence Strick (Research Director and Principle Investigator, Research Director and Principle Investigator, IBENS, PSL, CNRS, France)
This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.
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